【博士論文】学術データベース

博士論文 / The effect of transient oxygenation on stem cell mobilization and ischemia/reperfusion heart injury 高濃度酸素吸入が幹細胞動員と心臓虚血再灌流傷害に与える影響

著者

書誌事項

タイトル

The effect of transient oxygenation on stem cell mobilization and ischemia/reperfusion heart injury

タイトル別名

高濃度酸素吸入が幹細胞動員と心臓虚血再灌流傷害に与える影響

著者名

矢野倫太郎

学位授与大学

Nagasaki University (長崎大学) (大学ID:0073) (CAT機関ID:KI000877)

取得学位

博士(医学)

学位授与番号

甲医歯薬第1088号

学位授与年月日

2018-09-05

注記・抄録

For general anesthesia, pre-oxygenation is routinely performed prior to intubation. It is well-known that ischemic/hypoxic preconditioning induces stem cell mobilization and protects against ischemia/reperfusion (I/R) injury. In this study, we investigated the effect of transient oxygenation on stem cell mobilization and I/R injury of the heart. Mice were exposed to 100% oxygen for 5 or 20 minutes. We evaluated the number of c-kit+ stem/progenitor cells and the levels of SDF-1α and VEGF in peripheral blood at 1, 3, 6, and 24 hours after oxygenation. We also induced I/R injury of the heart at 3 hours post-oxygenation for 5 minutes and then examined stem cell recruitment and fibrotic changes in the heart 3 or 14 days later. The number of c-kit+ cells in peripheral blood was significantly increased at 1 or 24 hours after oxygenation for either 5 or 20 minutes. Oxygenation for 5 or 20 minutes did not significantly change the SDF-1α level measured in plasma. However, the plasma VEGF level was decreased at 3 hours post-oxygenation for 20 minutes (p = 0.051). Oxygenation for 5 minutes did not significantly alter the fibrotic area or cell apoptosis. Although oxygenation for 5 minutes increased the number of c-kit+ cells in hearts damaged by I/R injury, this difference was not significant between groups due to large variation between individuals (p = 0.14). Although transient oxygenation induces stem cell mobilization, it does not appear to protect against I/R injury of the heart in mice.

開始ページ : e0192733

元資料の権利情報 : © 2018 Yano et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

各種コード

NII論文ID(NAID)

500001081113

NII著者ID(NRID)
  • 8000001205371
DOI (出版社)

10.1371/journal.pone.0192733

DOI

info:doi/10.1371/journal.pone.0192733

本文言語コード

eng

データ提供元

機関リポジトリ / NDLデジタルコレクション

DOI

博士論文 / 長崎大学 / 医学

博士論文 / 長崎大学

博士論文 / 医学

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