【博士論文】学術データベース

博士論文 / An elevated expression of serum exosomal microRNA-191, - 21, -451a of pancreatic neoplasm is considered to be efficient diagnostic marker. 血清エクソソーム中microRNA-191,-21,-451aは膵腫瘍の有用な診断マーカーとなり得る

著者

書誌事項

タイトル

An elevated expression of serum exosomal microRNA-191, - 21, -451a of pancreatic neoplasm is considered to be efficient diagnostic marker.

タイトル別名

血清エクソソーム中microRNA-191,-21,-451aは膵腫瘍の有用な診断マーカーとなり得る

著者名

後藤拓磨

学位授与大学

旭川医科大学 (大学ID:0006) (CAT機関ID:KI000061)

取得学位

博士(医学)

学位授与番号

乙第473号

学位授与年月日

2018-06-29

注記・抄録

BACKGROUND:Pancreatic cancer is associated with an extremely poor prognosis, so new biomarkers that can detect the initial stages are urgently needed. The significance of serum microRNA (miR) levels in pancreatic neoplasm such as pancreatic cancer and intraductal papillary mucinous neoplasm (IPMN) diagnosis remains unclear. We herein evaluated the usefulness of miRs enclosed in serum exosomes (ExmiRs) as diagnostic markers.METHODS:The ExmiRs from patients with pancreatic cancer (n = 32) or IPMN (n = 29), and patients without neoplasms (controls; n = 22) were enriched using ExoQuick-TC™. The expression of ExmiRs was evaluated using a next-generation sequencing analysis, and the selected three miRs through this analysis were confirmed by a quantitative real-time polymerase chain reaction.RESULTS:The expression of ExmiR-191, ExmiR-21 and ExmiR-451a was significantly up-regulated in patients with pancreatic cancer and IPMN compared to the controls (p < 0.05). A receiver operating characteristic curve analysis showed that the area under the curve and the diagnostic accuracy of ExmiRs were 5-20% superior to those of three serum bulky circulating miRs (e.g.; ExmiR-21: AUC 0.826, accuracy 80.8%. Circulating miR-21: AUC 0.653, accuracy 62.3%). In addition, high ExmiR-451a was associated with mural nodules in IPMN (p = 0.010), and high ExmiR-21 was identified as a candidate prognostic factor for the overall survival (p = 0.011, HR 4.071, median OS of high-ExmiR-21: 344 days, median OS of low-ExmiR-21: 846 days) and chemo-resistant markers (p = 0.022).CONCLUSIONS:The level of three ExmiRs can thus serve as early diagnostic and progression markers of pancreatic cancer and IPMN, and considered more useful markers than the circulating miRs (limited to these three miRs).

開始ページ : 116

キーワード

pancreatic cancer, MicroRNA-21, tumor marker, Exosome, Intraductal papillary mucinous neoplasm, microRNA-451a

各種コード

NII論文ID(NAID)

500001070263

NII著者ID(NRID)
  • 8000001181612
DOI (出版社)

10.1186/s12885-018-4006-5.

本文言語コード

eng

データ提供元

機関リポジトリ / NDLデジタルコレクション

博士論文 / 旭川医科大学 / 医学

博士論文 / 旭川医科大学

博士論文 / 医学

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