【博士論文】学術データベース

博士論文 / Adipose-Derived Stem Cells and Vascularized Lymph Node Transfers Successfully Treat Mouse Hindlimb Secondary Lymphedema by Early Re-connection of the Lymphatic System and Lymphangiogenesis マウス下肢リンパ浮腫モデルにおける脂肪由来幹細胞と血管柄付きリンパ節移植の併用療法の効果

著者

書誌事項

タイトル

Adipose-Derived Stem Cells and Vascularized Lymph Node Transfers Successfully Treat Mouse Hindlimb Secondary Lymphedema by Early Re-connection of the Lymphatic System and Lymphangiogenesis

タイトル別名

マウス下肢リンパ浮腫モデルにおける脂肪由来幹細胞と血管柄付きリンパ節移植の併用療法の効果

著者名

林田健志

学位授与大学

Nagasaki University (長崎大学) (大学ID:0073) (CAT機関ID:KI000877)

取得学位

博士(医学)

学位授与番号

甲医歯薬第888号

学位授与年月日

2016-09-07

注記・抄録

Background: Secondary lymphedema is often observed in postmalignancy treatment of the breast and the gynecologic organs, but effective therapies have not been established in chronic cases even with advanced physiologic operations. Currently, reconstructive surgery with novel approaches has been attempted. Methods: The hindlimbs of 10-week-old male C57BL/6J mice, after 30-Gy x-irradiation, surgical lymph node dissection, and 5-mm gap creation, were divided into four groups, with vascularized lymph node transfer abdominal flap and 1.0 × 104 adipose-derived stem cells. Lymphatic flow assessment, a water-displacement plethysmometer paw volumetry test, tissue quantification of lymphatic vessels, and functional analysis of lymphatic vessels and nodes were performed. Results: Photodynamic Eye images, using indocyanine green fluorescence, demonstrated immediate staining in subiliac lymph nodes, and linear pattern imaging of the proximal region was observed with the combined treatment of adipose-derived stem cells and vascularized lymph node transfer. Both percentage improvement and percentage deterioration with the combined treatment of adipose-derived stem cells and vascularized lymph node transfer were significantly better than with other treatments (p < 0.05). The numbers of lymphatic vessels with LYVE-1 immunoreactivity significantly increased in mice treated with adipose-derived stem cells (p < 0.05), and B16 melanoma cells were metastasized in groups treated with vascularized lymph node transfers by day 28. Conclusions: Adipose-derived stem cells increase the number of lymphatic vessels and vascularized lymph node transfers induce the lymphatic flow drainage to the circulatory system. Combined adipose-derived stem cell and vascularized lymph node transfer treatment in secondary lymphedema may effectively decrease edema volume and restore lymphatic function by lymphangiogenesis and the lymphatic-to-venous circulation route.

開始ページ : 639

終了ページ : 651

元資料の権利情報 : © 2017 by the American Society of Plastic Surgeons.

元資料の権利情報 : This is a non-final version of an article published in final form in Plastic and Reconstructive Surgery, 139(3), pp.639-651; 2017.

各種コード

NII論文ID(NAID)

500001054426

NII著者ID(NRID)
  • 8000001059163
DOI (出版社)

10.1097/PRS.0000000000003110

DOI

info:doi/10.1097/PRS.0000000000003110

本文言語コード

eng

データ提供元

機関リポジトリ / NDLデジタルコレクション

DOI

博士論文 / 長崎大学 / 医学

博士論文 / 長崎大学

博士論文 / 医学

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