【博士論文】学術データベース

博士論文 / MicroRNA-130a is highly expressed in the esophageal mucosa of esophageal achalasia. 食道アカラシア患者の食道粘膜においてmicroRNA130a 発現は亢進している

著者

書誌事項

タイトル

MicroRNA-130a is highly expressed in the esophageal mucosa of esophageal achalasia.

タイトル別名

食道アカラシア患者の食道粘膜においてmicroRNA130a 発現は亢進している

著者名

庄司寛之

学位授与大学

Nagasaki University (長崎大学) (大学ID:0073) (CAT機関ID:KI000877)

取得学位

博士(医学)

学位授与番号

甲医歯薬第852号

学位授与年月日

2016-03-18

注記・抄録

Esophageal achalasia is considered as a risk factor of esophageal cancer. The etiologies of esophageal achalasia remain unknown. Peroral endoscopic myotomy (POEM) has recently been established as a minimally invasive method with high curability. The aims of the present study were to identify the microRNAs (miRs) specific to esophageal achalasia, to determine their potential target genes and to assess their alteration following POEM. RNA was extracted from biopsy samples from middle esophageal mucosa and analyzed using a microarray. Differentially expressed miRs in achalasia patients compared with control samples were identified and analyzed using reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR). Correlations between specific miR expression levels and the patients' clinical background were also investigated. In addition, alterations of selected miR expression levels before and after POEM were analyzed. The results of RT‑qPCR analysis demonstrated that the miR‑130a expression levels were significantly higher in patients with achalasia (P<0.0001). In addition, miR‑130a expression was significantly correlated with male sex and smoking history in patients with achalasia. However, no significant change in miR‑130a expression was observed between before and after POEM. In conclusion, miR‑130a is highly expressed in the esophageal mucosa of patients with achalasia and may be a biomarker of esophageal achalasia.

開始ページ : 898

終了ページ : 904

元資料の権利情報 : © Shoji et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

キーワード

esophageal cancer, microRNA, peroral endoscopic myotomy, esophageal achalasia, etiology

各種コード

NII論文ID(NAID)

500001054799

NII著者ID(NRID)
  • 8000001004895
DOI (出版社)

10.3892/etm.2017.4598

DOI

info:doi/10.3892/etm.2017.4598

本文言語コード

eng

データ提供元

機関リポジトリ / NDLデジタルコレクション

DOI

博士論文 / 長崎大学 / 医学

博士論文 / 長崎大学

博士論文 / 医学

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