【博士論文】学術データベース

博士論文 / Development and progression of immobilization-induced skin fibrosis through overexpression of transforming growth factor-ß1 and hypoxic conditions in a rat knee joint contracture model. TGF-ß1の過剰発現と低酸素状態は不動に伴う皮膚の線維化の発生と進行に関与する―膝関節屈曲拘縮モデルラットにおける検討―

著者

書誌事項

タイトル

Development and progression of immobilization-induced skin fibrosis through overexpression of transforming growth factor-ß1 and hypoxic conditions in a rat knee joint contracture model.

タイトル別名

TGF-ß1の過剰発現と低酸素状態は不動に伴う皮膚の線維化の発生と進行に関与する―膝関節屈曲拘縮モデルラットにおける検討―

著者名

後藤響

学位授与大学

Nagasaki University (長崎大学) (大学ID:0073) (CAT機関ID:KI000877)

取得学位

博士(医学)

学位授与番号

甲医歯薬第953号

学位授与年月日

2017-03-21

注記・抄録

Purpose: The purpose of this study was to investigate the pathology and mechanism of immobilization-induced skin fibrosis in a rat joint contracture model. Methods: Rats were randomly divided into control and immobilization groups. In the immobilization groups, knee joints of the rats were immobilized for 1, 2, and 4 weeks. After each immobilization, skin was dissected. To assess fibrosis in the skin, the thickness and area of adipocytes and connective tissue fibers were measured. Myofibroblasts were analyzed by immunohistochemistry by using anti-α-SMA as a marker. Gene expression levels of type I and III collagen, TGF-ß1, and HIF-1α were measured by using RT-PCR. Results: One week after immobilization, there was a marked increase in the area of connective tissue fibers in the immobilization group. Type I and type III collagen were significantly increased with prolonged immobilization. Higher numbers of α-SMA-positive cells were noted in the immobilized group at 2 and 4 weeks after immobilization. The expression level of TGF-β1 mRNA in the immobilization group increased after one week of immobilization. In contrast, the expression level of HIF1-α mRNA increased after 2 weeks of immobilization, and a greater increase was seen at 4 weeks after immobilization. Conclusions: These results suggest that immobilization induces skin fibrosis with accumulation of types I and III collagen. These fibrotic changes may be evoked by upregulation of TGF-β1 after one week of immobilization. Additionally, upregulation of HIF-1α may relate to skin fibrosis by accelerating the differentiation of fibroblasts to myofibroblasts starting at 2 weeks after immobilization.

開始ページ : 586

終了ページ : 596

元資料の権利情報 : © 2017 Taylor & Francis

元資料の権利情報 : This is an Accepted Manuscript of an article published by Taylor & Francis in Connective Tissue Research on 26 Feb 2017, available online: http://www.tandfonline.com/10.1080/03008207.2017.1284823.

キーワード

myofibroblast, hypoxia, skin fibrosis, Immobilization, collagen, transforming growth factor-β1

各種コード

NII論文ID(NAID)

500001054812

NII著者ID(NRID)
  • 8000001121830
DOI (出版社)

10.1080/03008207.2017.1284823

DOI

info:doi/10.1080/03008207.2017.1284823

本文言語コード

eng

データ提供元

機関リポジトリ / NDLデジタルコレクション

DOI

博士論文 / 長崎大学 / 医学

博士論文 / 長崎大学

博士論文 / 医学

関連著者

博士論文 / 大学

博士論文 / 学位