【博士論文】学術データベース

博士論文 / Type-1 polarized dendritic cells are a potent immunogen against Mycobacterium tuberculosis タイプ1免疫誘導型樹状細胞は結核菌に対して強力な免疫原性を有する

著者

書誌事項

タイトル

Type-1 polarized dendritic cells are a potent immunogen against Mycobacterium tuberculosis

タイトル別名

タイプ1免疫誘導型樹状細胞は結核菌に対して強力な免疫原性を有する

著者名

佐竹康臣

著者名

Satake Yasuomi

学位授与大学

浜松医科大学 (大学ID:0079) (CAT機関ID:KI000480)

取得学位

博士(医学)

学位授与番号

甲第746号

学位授与年月日

2017-05-01

注記・抄録

OBJECTIVE: Application of immunotherapy using dendritic cells (DCs) is considered an effective treatment strategy against persistent Mycobacterium tuberculosis infection. With the goal of developing improved therapeutic vaccination strategies for patients with tuberculosis (TB), we tested the ability of ex vivo-generated DCs to induce an effective TB antigen-specific type-1 immune response. METHODS: Monocyte-derived DCs from TB patients were induced to mature using a “standard” cytokine cocktail (IL-1β, TNF-α, IL-6, and PGE2) or a type 1-polarized DC (DC1) cocktail (IL-1β, TNF-α, IFN-α, IFN-γ, and polyinosinic:polycytidylic acid), and were loaded with the established TB antigen 6-kDa early secretory antigenic target protein (ESAT-6). RESULTS: Although DC1s from TB patients expressed the same levels of multiple co-stimulatory molecules (CD83, CD86, CD80, CD11c, and CD40) as the standard DCs (sDCs), DC1s secreted substantially higher levels of IL-12p70. Furthermore, when DCs pulsed with or without ESAT-6 were cultured with lymphocytes from the same patients, DC1s induced much higher numbers of ESAT-6-specific IFN-γ-producing T cells than sDCs, as manifested by their superior induction of natural killer cells activation and antigen-independent suppression of regulatory T cells. CONCLUSION: TB antigen-loaded DC1s are potent inducers of antigen-specific T cells, which could be used to develop improved immunotherapies of TB.

浜松医科大学学位論文 医博第746号(平成29年3月13日)

開始ページ : 523

終了ページ : 530

PubMed番号 : info:pmid/28399967

キーワード

IFN-γ, vaccine, IL-12

各種コード

NII論文ID(NAID)

500001054985

NII著者ID(NRID)
  • 8000001130141
  • 8000001130142
DOI (出版社)

10.5588/ijtld.16.0371

DOI

info:doi/10.5588/ijtld.16.0371

本文言語コード

eng

データ提供元

機関リポジトリ / NDLデジタルコレクション

DOI

博士論文 / 浜松医科大学 / 医学

博士論文 / 浜松医科大学

博士論文 / 医学

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