【博士論文】学術データベース

博士論文 / Understanding Physiological Dynamics of Periodontal Pathogens and Potential Probiotics, Lactobacillus spp. to Prevent Periodontal Disease 歯周病抑制のための歯周病原菌とプロバイオティクス菌乳酸菌群の生理動態の理解

著者

書誌事項

タイトル

Understanding Physiological Dynamics of Periodontal Pathogens and Potential Probiotics, Lactobacillus spp. to Prevent Periodontal Disease

タイトル別名

歯周病抑制のための歯周病原菌とプロバイオティクス菌乳酸菌群の生理動態の理解

著者名

Norzawani Jaffar

学位授与大学

九州工業大学 (大学ID:0071) (CAT機関ID:KI000844)

取得学位

博士(工学)

学位授与番号

甲生工第276号

学位授与年月日

2016-12-27

注記・抄録

Combinations of periodontal infection and destructive immune response are contributive factors to aggressive periodontitis and gingivitis, resulting in a poor prognosis to periodontal disease (Pihlstrom et al. 2005). Probiotic application using lactobacilli can be promising to counteract the periodontal disease. Probiotic owns unique properties and expresses beneficial roles in improving systemic health and also reducing the incidence of dental caries (Haukioja 2010). Hence, the main purpose of my Ph.D. study is to evaluate the dynamic of probiotic lactobacilli in the interaction with the periodontal pathogen and innate immune cells in denoting a positive role to prevent the periodontal disease. In this study, dynamic of periodontal pathogens and probiotic lactobacilli were evaluated via the following 4 approaches; 1) Inhibitive interaction between probiotic lactobacilli and periodontal pathogens via direct and indirect co-culture approaches, 2) Counteraction for the mature biofilm of the periodontal pathogens, Aggregatibacter actinomycetemcomitans strains by the potential probiotic lactobacilli, 3) understanding the biofilm formation of periodontal pathogens on hydroxyapatite surfaces, and 4) activation of phagocytic function by probiotic lactobacilli in combating A. actinomycetemcomitans infection. Our finding shows, 1) different interaction of inhibition was found in the direct and indirect co-culture method, by which significant bactericidal activity by certain species of probiotic lactobacilli was observed in the indirect approach, whereas direct co-culture showed vice versa, 2) probiotic lactobacilli were potently degrading the matured formed-biofilm of A. actinomycetemcomitans strains, 3) varied effect of biofilm formation on the hydroxyapatite surfaces were observed between two periodontal pathogen species incorporated with complex calcium intake, 4) probiotic lactobacilli showed the potential to induce the murine macrophage polarization prior A. actinomycetemcomitans Y4 infection. In conclusion, probiotic lactobacilli has found to own beneficial role against the periodontal pathogens activity and modulate the macrophage activity toward prevention of periodontal disease. This findings are indeed important in providing essential information on the physiological dynamics of periodontal pathogen and probiotic lactobacilli, meanwhile characterized the new insight into how probiotic lactobacilli roles to counteract the activities of the periodontal pathogen and modulate the innate immune system towards a preventive mode of periodontal disease.

九州工業大学博士学位論文 学位記番号:生工博甲第276号 学位授与年月日:平成28年12月27日

1 Introduction and literature review| 2 General materials and methods| 3 Interaction of probiotic bacteria with periodontal pathogen| 4 Mature biofilm degradation by potential probiotics: Aggregatibacter actinomycetemcomitans VERSUS Lactobacillus spp.| 5 Biofilm formation of periodontal pathogens on hydroxyapatite surfaces: implications for periodontium damage| 6 Probiotic induce phagocytic killing of A. actinomycetemcomitans Y4 in raw264.7 murine macrophage cells| 7 Concluding remaks and suggestion for future research

平成28年度

九州工業大学博士学位論文(要旨)学位記番号:生工博甲第276号 学位授与年月日:平成28年12月27日

キーワード

probiotics, Periodontal pathogens Lactobacillus

各種コード

NII論文ID(NAID)

500001052308

NII著者ID(NRID)
  • 8000001099460
本文言語コード

eng

データ提供元

機関リポジトリ / NDLデジタルコレクション

博士論文 / 九州工業大学 / 工学

博士論文 / 九州工業大学

博士論文 / 工学

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